Intraspecies Genomic Diversity and Natural Population Structure of the Meat-Borne Lactic Acid Bacterium <i>Lactobacillus sakei</i>
Stéphane Chaillou, Marie Daty, Fabienne Baraige, Anne-Marie Dudez, Patricia Anglade, Rhys J. Jones +3 more
Applied and Environmental Microbiology
Abstract
Lactobacillus sakei is a food-borne bacterium naturally found in meat and fish products. A study was performed to examine the intraspecies diversity among 73 isolates sourced from laboratory collections in several different countries. Pulsed-field gel electrophoresis analysis demonstrated a 25% variation in genome size between isolates, ranging from 1,815 kb to 2,310 kb. The relatedness between isolates was then determined using a PCR-based method that detects the possession of 60 chromosomal genes belonging to the flexible gene pool. Ten different strain clusters were identified that had noticeable differences in their average genome size reflecting the natural population structure. The results show that many different genotypes may be isolated from similar types of meat products, suggesting a complex ecological habitat in which intraspecies diversity may be required for successful adaptation. Finally, proteomic analysis revealed a slight difference between the migration patterns of highly abundant GapA isoforms of the two prevailing L. sakei subspecies (sakei and carnosus). This analysis was used to affiliate the genotypic clusters with the corresponding subspecies. These findings reveal for the first time the extent of intraspecies genomic diversity in L. sakei. Consequently, identification of molecular subtypes may in the future prove valuable for a better understanding of microbial ecosystems in food products.
Extracted Claims
4 claims extracted from this paper into the knowledge graph
Lactobacillus sakei is found meat and fish products
“Lactobacillus sakei is a food-borne bacterium naturally found in meat and fish products.”
Lactobacillus sakei shows proteomic differences
“proteomic analysis revealed a slight difference between the migration patterns of highly abundant GapA isoforms of the two prevailing L. sakei subspecies (sakei and carnosus).”
Lactobacillus sakei has genotypic clusters
“Ten different strain clusters were identified that had noticeable differences in their average genome size reflecting the natural population structure.”