Enumeration of Vibrio cholerae O1 in Bangladesh waters by fluorescent-antibody direct viable count
P R Brayton, Mark L. Tamplin, Anwar Huq, Rita R. Colwell
Applied and Environmental Microbiology
Abstract
A field trial to enumerate Vibrio cholerae O1 in aquatic environments in Bangladesh was conducted, comparing fluorescent-antibody direct viable count with culture detection by the most-probable-number index. Specificity of a monoclonal antibody prepared against the O1 antigen was assessed and incorporated into the fluorescence staining method. All pond and water samples yielded higher counts of viable V. cholerae O1 by fluorescent-antibody direct viable count than by the most-probable-number index. Fluorescence microscopy is a more sensitive detection system than culture methods because it allows the enumeration of both culturable and nonculturable cells and therefore provides more precise monitoring of microbiological water quality.
Extracted Claims
2 claims extracted from this paper into the knowledge graph
Vibrio cholerae O1 enumerated aquatic environments
“A field trial to enumerate Vibrio cholerae O1 in aquatic environments in Bangladesh was conducted, comparing fluorescent-antibody direct viable count with culture detection by the most-probable-number...”
fluorescent-antibody direct viable count yielded higher counts
“All pond and water samples yielded higher counts of viable V. cholerae O1 by fluorescent-antibody direct viable count than by the most-probable-number index.”