AIE-active UiO-66@PtNPs nanozyme-amplified fluorescence and colorimetric dual-signal magnetic immunosensing platform for effective detection of aflatoxin B1 in food products.

Abstract

Highly toxic aflatoxin B1 (AFB1) frequently contaminates food products, posing a serious threat to human health. Developing accurate, efficient and user-friendly detection strategies is essential. This study synthesized a novel H4TCPE-UiO-66@PtNPs nanozyme with stable fluorescence and catalytic properties through integrating H4TCPE ligands possessing aggregation-induced luminescence (AIE) properties with in-situ grown PtNPs, and further developed a fluorescence-colorimetric dual-signal immunosensing platform for the detection of AFB1 contamination in food products. Utilizing an immunocompetitive reaction and magnetic separation techniques, the platform enabled quantitative analysis of AFB1, achieving favorable linear range of 0.05-100 ng/mL and high sensitivity (fluorescence LOD: 0.022 ng/mL, colorimetric LOD: 0.047 ng/mL). Real samples tests yielded satisfactory recoveries (84.6%-110.1%) and good reproducibility (RSD ≤ 5.3%, n = 3), demonstrating the platform's potential for specific identification and screening of AFB1 in complex matrices. The proposed dual-signal detection strategy provides an effective solution for self-calibrated and rapid analysis of foodborne or environmental pollutants.