Rapid detection of Alicyclobacillus acidoterrestris in juice using an electrochemical immunosensor: Correlation with growth kinetics and spoilagez potential.

Abstract

Alicyclobacillus acidoterrestris (A. acidoterrestris) is the primary spoilage microorganism in apple juice, producing guaiacol (GUA) through metabolism, which results in characteristic off-flavors and cloudiness. This study established a comprehensive quality assessment method using GC-IMS, relative odor activity value (ROAV), Logistic modeling, sensory evaluation and Pearson correlation analysis, which demonstrated that quantitative detection of A. acidoterrestris in fruit juice enables reliable prediction of spoilage. An electrochemical immunosensor was developed by immobilizing anti-A. acidoterrestris monoclonal antibody (mAb) onto NiMnSe/SWNTs@PPy nanocomposite-modified electrodes, enabling highly sensitive detection (linear detection range: 10-1 × 107 CFU/mL; limit of detection (LOD): 3.45 CFU/mL; recovery: 97.66%-110.16%; relative standard deviation (RSD): 2.05%-6.26%). Additionally, the logistic model enabled the sensor to predict the degree of flavor deterioration in juice. This dual-function tool provides simultaneous A. acidoterrestris detection and quality prediction, offering significant advantages for juice quality control.