Hollow zeolitic imidazolate framework-8 anchored on reduced graphene oxide aerogel as adsorbent of solid-phase extraction coupled with high-performance liquid chromatography for determination of ciprofloxacin in egg.

Abstract

The development of adsorbents in sample pretreatment technologies for detecting fluoroquinolone antibiotic residues remains a critical challenge in ensuring food safety. A hollow ZIF-8/reduced graphene oxide aerogel (HRA) is developed as an adsorbent of solid-phase extraction (SPE) in combination with high performance liquid chromatography (HPLC) to realize the efficient enrichment and trace detection of ciprofloxacin (CIP) in egg. Etched hollow ZIF-8 provides unsaturated Zn metal sites and abundant functional groups to specifically recognize CIP. Hollow ZIF-8 loaded onto reduced graphene oxide aerogel displays favorable formability and hierarchical structure, effectively improving the mass transfer effect. An exceptional adsorption capacity of CIP up to 893.1 mg·g-1 realizes the effective pre-enrichment of CIP, with a low limit of detection (LOD) and limit of quantification (LOQ) are 0.042 μg·L-1 and 0.14 μg·L-1, respectively. Satisfactory recovery rates of 90.7 %-108.9 % with RSD<3.4 % are obtained in detecting CIP residues in egg samples. This study provides a promising approach for detecting antibiotic residues in eggs to ensure food safety.