Optimization of the detection of enteric viruses in mussels by real-time PCR / RT-PCR.
Siméon Goïta, Hibatallah Lachkar, Nouhaila Elfellaki, Houda Rafi, Abdelfattah Mouabad, Frank Le Foll +2 more
Journal of microbiological methods
Abstract
Enteric viruses, including adenovirus, norovirus, and rotavirus, represent a significant threat to public health due to their potential to cause foodborne illnesses. The aim of this study was to enhance the sensitivity of the method ISO 15216-2:2019, by introducing an additional virus concentration step based on polyethylene glycol precipitation followed by a centrifugation.