Metatranscriptomics-guided discovery and characterization of a polyphenol-metabolizing gut microbial enzyme
Minwoo Bae, Chi Hieu Le, Raaj S. Mehta, Xueyang Dong, Lindsey M. Pieper, Lorenzo Ramirez +6 more
Cell Host & Microbe
Abstract
Gut microbial catechol dehydroxylases are a largely uncharacterized family of metalloenzymes that potentially impact human health by metabolizing dietary polyphenols. Here, we use metatranscriptomics (MTX) to identify highly transcribed catechol-dehydroxylase-encoding genes in human gut microbiomes. We discover a prevalent, previously uncharacterized catechol dehydroxylase (Gp Hcdh) from Gordonibacter pamelaeae that dehydroxylates hydrocaffeic acid (HCA), an anti-inflammatory gut microbial metabolite derived from plant-based foods. Further analyses suggest that the activity of Gp Hcdh may reduce anti-inflammatory benefits of polyphenol-rich foods. Together, these results show the utility of combining MTX analysis and biochemical characterization for gut microbial enzyme discovery and reveal a potential link between host inflammation and a specific polyphenol-metabolizing gut microbial enzyme.
Extracted Claims
2 claims extracted from this paper into the knowledge graph
catechol dehydroxylase dehydroxylates hydrocaffeic acid
“We discover a prevalent, previously uncharacterized catechol dehydroxylase (Gp Hcdh) from Gordonibacter pamelaeae that dehydroxylates hydrocaffeic acid (HCA), an anti-inflammatory gut microbial metabo...”
catechol dehydroxylase activity reduces anti-inflammatory benefits of polyphenol-rich foods
“Further analyses suggest that the activity of Gp Hcdh may reduce anti-inflammatory benefits of polyphenol-rich foods.”