In Vitro Evaluation of the Most Active Probiotic Strains Able to Improve the Intestinal Barrier Functions and to Prevent Inflammatory Diseases of the Gastrointestinal System

Abstract

<i>Background</i>: The integrity of the intestinal barrier is fundamental to gut health and homeostasis; its damage can increase intestinal permeability, with translocation of bacteria and/or endotoxins from gut, and the onset of various intestinal diseases. <i>Lactobacillus</i> spp. is one of the most common probiotics normally found in fermented foods and dairy products and is known for its anti-inflammatory and immunomodulatory properties and for its ability to protect and enhance the intestinal barrier functions. The aim of this work was to evaluate the ability of different strains of <i>Lactobacillus</i> spp. to improve in vitro the integrity of the intestinal barrier, to exert anti-inflammatory and immunomodulatory activity and to prevent <i>Salmonella</i> Typhimurium and enteroinvasive <i>Escherichia coli</i> (EIEC) infections. <i>Methods</i>: We analyzed the cellular expression of tight junctions, antimicrobial peptide HBD-2, pro-inflammatory cytokines and the inhibition of pathogens adhesion and invasion in a model of co-cultured epithelial cells treated with <i>Lactobacillus</i> spp. <i>Results</i>: <i>L. brevis, L. reuteri</i> and <i>L. rhamnosus</i> proved to be more effective in protecting the intestinal epithelium. <i>Conclusions:</i> These in vitro studies can help select strains particularly active in their intended use to obtain consortia formulations that can have as much maximum yield as possible in terms of patient benefit.