Optimization of Extraction Conditions to Improve Chlorogenic Acid Content and Antioxidant Activity of Extracts from Forced Witloof Chicory Roots
Morad Chadni, Emilie Isidore, Étienne Diemer, Otmane Ouguir, Fanny Brunois, Régis Catteau +2 more
Foods
Abstract
Chlorogenic acids are major phenolic constituents in many herbal medicines and exhibit various bioactivities that explain the growing interest in extracting chlorogenic acids from biomass. In this context, the present study aims to maximize 3-<i>O</i>-Caffeoylquinic acid (3-CQA) and 3,5-<i>O</i>-di-caffeoylquinic acid (3,5-diCQA) contents from forced witloof chicory roots and to analyze the extraction kinetic modelling. First, the solid-liquid ratio, ethanol concentration, extraction time and temperature were studied. The extraction conditions were optimized to maximize the extraction of these compounds. The maximum yields reached 5 ± 0.11 and 5.97 ± 0.30 mg/g dry matter (DM) for 3-<i>O</i>-Caffeoylquinic acid and 3,5-<i>O</i>-di-caffeoylquinic acid, respectively, in less than 6 min at 70 °C. Extraction with water as a solvent was assessed with the aim of proposing a second greener and less-expensive solvent. This extraction is very fast from 90 °C, with a maximum of 6.22 ± 0.18 mg/g<sub>DM</sub> of 3-<i>O</i>-Caffeoylquinic acid, and instantaneous for 3,5-<i>O</i>-di-caffeoylquinic acid with a maximum of 6.44 ± 0.59 mg/g<sub>DM</sub>. In the second step, response surface methodology was employed to optimize the ultrasound-assisted extraction of antioxidants. The higher antioxidant activities were found at temperatures from 40 °C and at percentages of ethanol in the range of 35-70%.
Extracted Claims
5 claims extracted from this paper into the knowledge graph
ultrasound-assisted extraction of antioxidants optimized higher antioxidant activities
“In the second step, response surface methodology was employed to optimize the ultrasound-assisted extraction of antioxidants. The higher antioxidant activities were found at temperatures from 40 °C an...”
extraction of 3,5-O-di-caffeoylquinic acid optimized 5.97 ± 0.30 mg/g dry matter
“The maximum yields reached 5 ± 0.11 and 5.97 ± 0.30 mg/g dry matter (DM) for 3-O-Caffeoylquinic acid and 3,5-O-di-caffeoylquinic acid, respectively, in less than 6 min at 70 °C.”
extraction of 3-O-Caffeoylquinic acid optimized 6.22 ± 0.18 mg/g DM
“Extraction with water as a solvent was assessed with the aim of proposing a second greener and less-expensive solvent. This extraction is very fast from 90 °C, with a maximum of 6.22 ± 0.18 mg/g DM of...”