Identification and verification of key taste components in wampee using widely targeted metabolomics
Qingchun Yin, Jian-bang Ji, Rong-hu Zhang, Zhouwei Duan, Hui Xie, Zhe Chen +2 more
Food Chemistry X
Abstract
Due to the lack of comprehensive evaluation of all metabolites in wampee, the metabolic reasons for taste differences are unclear. Here, two local varieties YF1 (sweet taste) and YF2 (sweet-sour taste), were selected for quality analysis, followed by ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) based widely targeted metabolomic analysis. YF1 and YF2 were clearly separated by principal component analysis (PCA) and cluster analysis, and 449 metabolites were different between the cultivars, including 29 carbohydrates and 29 organic acids. Among them, d-galactose, d-mannose, and d-fructose 6-phosphate contributed mainly to the sweet taste of the YF1 wampee. l-citramalic acid, 2-hydroxyglutaric acid, and 3-methylmalic acid were the dominant organic acids in YF2 wampee, and therefore, contributed primarily to the sweet-sour taste. The differential metabolites were significantly enriched in the "ascorbate and aldarate metabolism" and "C5-branched dibasic acid metabolism" pathways. Ascorbate played a crucial role in the regulation of sugars and organic acids through those pathways. In addition, high-performance liquid chromatography (HPLC) based quantitative verification exhibited the same specific cultivar variations.
Extracted Claims
3 claims extracted from this paper into the knowledge graph
d-galactose, d-mannose, and d-fructose 6-phosphate contribute mainly to the sweet taste of the YF1 wampee
“Among them, d-galactose, d-mannose, and d-fructose 6-phosphate contributed mainly to the sweet taste of the YF1 wampee.”
l-citramalic acid, 2-hydroxyglutaric acid, and 3-methylmalic acid contribute primarily to the sweet-sour taste of the YF2 wampee
“l-citramalic acid, 2-hydroxyglutaric acid, and 3-methylmalic acid were the dominant organic acids in YF2 wampee, and therefore, contributed primarily to the sweet-sour taste.”
ascorbate plays a crucial role in the regulation of sugars and organic acids through the 'ascorbate and aldarate metabolism' and 'C5-branched dibasic acid metabolism' pathways
“Ascorbate played a crucial role in the regulation of sugars and organic acids through those pathways.”