Glycosyltransferase from <i>Bacteroides gallinaceum</i> Is a Novel α-1,3-Fucosyltransferase that Can Be Used for 3-Fucosyllactose Production In Vivo by Metabolically Engineered <i>Escherichia coli</i>
Geng Chen, Hao Wu, Yingying Zhu, Li Wan, Wenli Zhang, Wanmeng Mu
Journal of Agricultural and Food Chemistry
Abstract
As one of the attractive fucosylated human milk oligosaccharides, the biological production of 3-fucosyllactose (3-FL) has received great attention, as it exhibits many excellent physiological functions for infants. In this work, a novel 3-FL-producing α-1,3-fucosyltransferase (α1,3-FucT) named FutM2 from <i>Bacteroides gallinaceum</i> was first selected from nine potential candidates in the NCBI database. Then, a highly 3-FL-producing engineered <i>Escherichia coli</i> strain was constructed by modular pathway enhancement including the GDL-l-fucose precursor supply by overexpressing manC, manB, gmd, and wcaG (CBGW), and the 3-FL synthesis pathway by introducing <i>B. gallinaceum</i> FutM2. Finally, a titer of 20.3 g L<sup>-1</sup> and productivity of 0.40 g L<sup>-1</sup> h<sup>-1</sup> of 3-FL were achieved in the 3-L bioreactor by engineered <i>E. coli</i> (Δ<i>lacZ</i>Δ<i>wcaJ</i>) harboring pCDF-<i>CBGW</i> and pET-<i>futM2</i>. Our study provided a novel α1,3-FucT from <i>B. gallinaceum</i> that could be used for 3-FL production, presenting an efficient microbial cell factory platform to de novo synthesize 3-FL from glycerol.
Extracted Claims
3 claims extracted from this paper into the knowledge graph
engineered E. coli produces 3-FL
“Finally, a titer of 20.3 g L^-1 and productivity of 0.40 g L^-1 h^-1 of 3-FL were achieved in the 3-L bioreactor by engineered E. coli (ΔlacZΔwcaJ) harboring pCDF-CBGW and pET-futM2”
α-1,3-fucosyltransferase (α1,3-FucT) FutM2 is used for 3-FL production
“In this work, a novel 3-FL-producing α-1,3-fucosyltransferase (α1,3-FucT) named FutM2 from Bacteroides gallinaceum was first selected from nine potential candidates in the NCBI database”
3-fucosyllactose (3-FL) exhibits physiological functions for infants
“As one of the attractive fucosylated human milk oligosaccharides, the biological production of 3-fucosyllactose (3-FL) has received great attention, as it exhibits many excellent physiological functio...”