The Microbial Diversity and Biofilm-Forming Characteristic of Two Traditional Tibetan Kefir Grains
Xiaomeng Wang, Wenpei Li, Wenpei Li, Mengjia Xu, Juanjuan Tian, Wei Li +1 more
Foods
Abstract
In this study, a high-throughput sequencing technique was used to analyze bacterial and fungal diversity of two traditional Tibetan kefir grains from Linzhi (K1) and Naqu (K2) regions. Comparative bioinformatic analyses indicated that <i>Lactobacillus kefiranofaciens</i>, <i>L. kefiri</i> and <i>Kluyveromyces marxianus</i> were the main dominant strains in K1 and K2. In order to research the relationship of the growth of kefir grains, the biofilm and the extracellular polysaccharides (EPS) produced by microorganisms, the proliferation rate of kefir grains, the yield and chemical structure of EPS and the optimal days for biofilm formation were determined. The results showed that the growth rate, the yield of EPS and the biofilm formation ability of K1 were higher than K2, and the optimal day of their biofilm formation was the same in 10th day. Additionally, the live cells, dead cells and EPS in biofilm formation of K1 and K2 were observed by fluorescence microscope to clarify the formation process of kefir grains. To determine the influence of microbial interactions on biofilm and the formation of kefir grains, the essential role of microbial quorum sensing needs further attention.
Extracted Claims
7 claims extracted from this paper into the knowledge graph
biofilm formation occurs optimally on 10th day
“the optimal day of their biofilm formation was the same in 10th day.”
K1 has higher yield of EPS
“the growth rate, the yield of EPS and the biofilm formation ability of K1 were higher than K2”
Kluyveromyces marxianus is dominant in Tibetan kefir grains
“<i>Lactobacillus kefiranofaciens</i>, <i>L. kefiri</i> and <i>Kluyveromyces marxianus</i> were the main dominant strains in K1 and K2.”