Genetic tools for the development of recombinant lactic acid bacteria
Jiapeng Wu, Yongping Xin, Jian Kong, Tingting Guo
Microbial Cell Factories
Abstract
Lactic acid bacteria (LAB) are a phylogenetically diverse group with the ability to convert soluble carbohydrates into lactic acid. Many LAB have a long history of safe use in fermented foods and are recognized as food-grade microorganisms. LAB are also natural inhabitants of the human intestinal tract and have beneficial effects on health. Considering these properties, LAB have potential applications as biotherapeutic vehicles to delivery cytokines, antigens and other medicinal molecules. In this review, we summarize the development of, and advances in, genome manipulation techniques for engineering LAB and the expected future development of such genetic tools. These methods are crucial for us to maximize the value of LAB. We also discuss applications of the genome-editing tools in enhancing probiotic characteristics and therapeutic functionalities of LAB.
Extracted Claims
4 claims extracted from this paper into the knowledge graph
Lactic acid bacteria (LAB) convert soluble carbohydrates into lactic acid
“Lactic acid bacteria (LAB) are a phylogenetically diverse group with the ability to convert soluble carbohydrates into lactic acid.”
Lactic acid bacteria (LAB) have beneficial effects on health
“LAB are also natural inhabitants of the human intestinal tract and have beneficial effects on health.”
Lactic acid bacteria (LAB) are natural inhabitants of the human intestinal tract
“LAB are also natural inhabitants of the human intestinal tract and have beneficial effects on health.”