Physicochemical, functional, thermal and structural properties of isolated Kabuli chickpea proteins as affected by processing approaches
Yixiang Xu, Mary Obielodan, Edward Sismour, Anna Arnett, Saeedah Alzahrani, Baoshan Zhang
International Journal of Food Science & Technology
Abstract
Summary Kabuli chickpea seeds were processed by soaking only and soaking followed either by pressure cooking or roasting. Proteins were isolated from both raw and processed seeds, and the effects of these processing approaches on the physicochemical, functional, thermal and structural properties as well as SDS ‐ PAGE profiles of the protein isolates were investigated. Thermal processes significantly ( P < 0.05) decreased protein yield, content, colour difference, emulsifying properties and protein solubility of the protein isolates, but increased lightness and water and oil absorption capacities. Protein thermal properties, secondary structure and SDS ‐ PAGE banding patterns were significantly changed in thermal‐processed samples, especially those that were pressure cooked. No endothermic peak was detected in differential scanning calorimetry thermograms, and peak intensity of amide I absorption band at 1600–1700 cm −1 in Fourier transform infrared spectra reduced. The results reveal that pressure cooking had more pronounced effects on the properties of the protein isolates than other processing approaches.
Extracted Claims
3 claims extracted from this paper into the knowledge graph
pressure cooking decreases protein yield, content, colour difference, emulsifying properties, and protein solubility
“Thermal processes significantly (P < 0.05) decreased protein yield, content, colour difference, emulsifying properties and protein solubility of the protein isolates, but increased lightness and water...”
pressure cooking increases lightness and water and oil absorption capacities
“Thermal processes significantly (P < 0.05) decreased protein yield, content, colour difference, emulsifying properties and protein solubility of the protein isolates, but increased lightness and water...”
pressure cooking changes protein thermal properties, secondary structure, and SDS-PAGE banding patterns
“Protein thermal properties, secondary structure and SDS-PAGE banding patterns were significantly changed in thermal-processed samples, especially those that were pressure cooked.”