In vitro gastrointestinal digestion promotes the protective effect of blackberry extract against acrylamide-induced oxidative stress
Wei Chen, Hongming Su, Yang Xu, Chao Jin
Scientific Reports
Abstract
Acrylamide (AA)-induced toxicity has been associated with accumulation of excessive reactive oxygen species. The present study was therefore undertaken to investigate the protective effect of blackberry digests produced after (BBD) in vitro gastrointestinal (GI) digestion against AA-induced oxidative damage. The results indicated that the BBD (0.5 mg/mL) pretreatment significantly suppressed AA-induced intracellular ROS generation (56.6 ± 2.9% of AA treatment), mitochondrial membrane potential (MMP) decrease (297 ± 18% of AA treatment) and glutathione (GSH) depletion (307 ± 23% of AA treatment), thereby ameliorating cytotoxicity. Furthermore, LC/MS/MS analysis identified eight phenolic compounds with high contents in BBD, including ellagic acid, ellagic acid pentoside, ellagic acid glucuronoside, methyl-ellagic acid pentoside, methyl-ellagic acid glucuronoside, cyanidin glucoside, gallic acid and galloyl esters, as primary active compounds responsible for antioxidant action. Collectively, our study uncovered that the protective effect of blackberry was reserved after gastrointestinal digestion in combating exogenous pollutant-induced oxidative stress.
Extracted Claims
12 claims extracted from this paper into the knowledge graph
blackberry extract ameliorates AA-induced cytotoxicity
“thereby ameliorating cytotoxicity”
blackberry extract prevents AA-induced mitochondrial membrane potential decrease
“mitochondrial membrane potential (MMP) decrease (297 ± 18% of AA treatment)”
methyl-ellagic acid glucuronoside is present in blackberry extract
“LC/MS/MS analysis identified eight phenolic compounds with high contents in BBD, including methyl-ellagic acid glucuronoside”