Polyphenol‐Rich Beverages Enhance Zinc Uptake and Metallothionein Expression in Caco‐2 Cells
Sreenivasulu Kilari, Raghu Pullakhandam, K. Madhavan Nair
Journal of Food Science
Abstract
The effect of red wine (RW), red grape juice (RGJ), green tea (GT), and representative polyphenols on Caco-2 cell (65)Zn uptake was explored. RW, RGJ, and GT enhanced the uptake of zinc from rice matrix. Fractionation of RW revealed that enhancing activity of zinc uptake was exclusively resided in the polyphenol fraction. Among the polyphenols tested, only tannic acid and quercitin stimulated the uptake of zinc while others did not influence the uptake. In tune with these results, only tannic acid and quercitin competed with zinquin (a zinc selective fluorophore) for zinc in vitro. Although all the polyphenols tested appear to enhance the expression of metallothionein (MT), the induction was higher with tannic acid, quercitin, and RW extract. Furthermore, phytic acid abrogated the tannic acid-induced MT expression. These results suggest that polyphenol-rich beverages, tannic acid, and quercitin bind and stimulate the zinc uptake and MT expression in Caco-2 cells.
Extracted Claims
7 claims extracted from this paper into the knowledge graph
tannic acid, quercitin compete with zinquin for zinc in vitro
“In tune with these results, only tannic acid and quercitin competed with zinquin (a zinc selective fluorophore) for zinc in vitro.”
polyphenol fraction of red wine reside in enhancing activity of zinc uptake
“Fractionation of RW revealed that enhancing activity of zinc uptake was exclusively resided in the polyphenol fraction.”
red wine (RW), red grape juice (RGJ), green tea (GT) enhance zinc uptake from rice matrix
“RW, RGJ, and GT enhanced the uptake of zinc from rice matrix.”