Effects of Ethylene and 1-Methylcyclopropene (1-MCP) on Gene Expression and Activity Profile of α-1,4-Glucan-phosphorylase during Banana Ripening
Janaína Aparecida Mainardi, Eduardo Purgatto, Adair Vieira, Walter Arato Bastos, Beatriz Rosana Cordenunsi, João Roberto Oliveira do Nascimento +1 more
Journal of Agricultural and Food Chemistry
Abstract
Starch phosphorylases are enzymes that can use starch as substrate, and they are supposed to act in both in starch synthesis and degradation. This paper reports the effects of ethylene and 1-methylcyclopropene (1-MCP) on the degradation of starch and phosphorylase activity and gene expression. The results indicate that phosphorylase activity is induced during ripening and that it is associated with the onset of starch degradation. The regulation of banana phosphorylase activity is mainly dependent on gene expression, and the absence of ethylene perception by 1-MCP had a positive effect. However, this effect can be precluded by increased levels of ethylene, both autocatalytic and exogenous.
Extracted Claims
3 claims extracted from this paper into the knowledge graph
ethylene induces phosphorylase activity during banana ripening
“The results indicate that phosphorylase activity is induced during ripening and that it is associated with the onset of starch degradation.”
1-MCP has a positive effect phosphorylase activity
“The regulation of banana phosphorylase activity is mainly dependent on gene expression, and the absence of ethylene perception by 1-MCP had a positive effect.”
1-MCP precludes positive effect of 1-MCP on phosphorylase activity
“However, this effect can be precluded by increased levels of ethylene, both autocatalytic and exogenous.”