LC-DAD-ESI/MS<sup><i>n</i></sup> Determination of Direct Condensation Flavanol−Anthocyanin Adducts in Pressure Extracted Pomegranate (<i>Punica granatum</i> L.) Juice
Enrique Sentandreu, José Luis Navarro, José M. Sendra
Journal of Agricultural and Food Chemistry
Abstract
Pomegranate (Punica granatum L.) juice, obtained by pressure extraction of the whole fruit, has been analyzed for its flavanol-anthocyanin adduct content using reversed-phase liquid chromatography with diode array detection, coupled to mass spectrometry (ion trap) with electrospray ionization (HPLC-DAD-ESI/MS(n)), operating in positive ion mode. A total of 35 dimers have been detected, consisting of mono- and disubstituted hexoside derivatives of the adducts between the flavan-3-ols (epi)gallocatechin, (epi)catechin and (epi)afzelechin and the anthocyanidins delphinidin, cyanidin and pelargonidin. In addition, evidence is given for the presence of additional anthocyanin-flavanol adducts in this juice.
Extracted Claims
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flavanol-anthocyanin adducts detected in pomegranate juice
“A total of 35 dimers have been detected, consisting of mono- and disubstituted hexoside derivatives of the adducts between the flavan-3-ols (epi)gallocatechin, (epi)catechin and (epi)afzelechin and th...”