A Phytochemical Screening Procedure for Sweet Ent-Kaurene Glycosides in the Genus Stevia
A. Douglas Kinghorn, Djaja D. Soejarto, N. P. Dhammika Nanayakkara, César M. Compadre, Helena C. Makapugay, J. M. Hovanec-Brown +2 more
Journal of Natural Products
Abstract
Altogether, 110 species of the genus Stevia, comprising both herbarium and fresh leaf samples, were screened for the presence of sweet ent-kaurene glycosides, using a combination of tlc and hplc, followed by gc/ms. Stevioside and rebaudiosides A and C were detected in a Stevia rebaudiana herbarium specimen collected in Paraguay in 1919, and stevioside was observed as a constituent of a Stevia phlebophylla herbarium specimen collected in Mexico in 1889. Steviol glycosides were not detected in any of the other 108 Stevia species studied. The phytochemical results obtained in this study are correlated with those of preliminary organoleptic tests on the sweetness of these Stevia samples, and the chemotaxonomic implications of the present findings are discussed.
Extracted Claims
4 claims extracted from this paper into the knowledge graph
stevioside observed as a constituent of Stevia phlebophylla herbarium specimen
“Stevioside and rebaudiosides A and C were detected in a Stevia rebaudiana herbarium specimen collected in Paraguay in 1919, and stevioside was observed as a constituent of a Stevia phlebophylla herbar...”
rebaudiosides A and C detected in Stevia rebaudiana herbarium specimen
“Stevioside and rebaudiosides A and C were detected in a Stevia rebaudiana herbarium specimen collected in Paraguay in 1919, and stevioside was observed as a constituent of a Stevia phlebophylla herbar...”
stevioside detected in Stevia rebaudiana herbarium specimen
“Stevioside and rebaudiosides A and C were detected in a Stevia rebaudiana herbarium specimen collected in Paraguay in 1919, and stevioside was observed as a constituent of a Stevia phlebophylla herbar...”