Complete amino acid sequences of five dimeric and four monomeric forms of metallothionein from the edible mussel <i>Mytilus edulis</i>
Elaine A. Mackay, J. Overnell, B. Dunbar, Ian Davidson, Peter Hunziker, Jeremias H.R. Kägi +1 more
European Journal of Biochemistry
Abstract
Cadmium-induced metallothioneins from the common sea mussel, Mytilus edulis, were shown to comprise of two groups of isoforms having apparent molecular masses of 10 kDa and 20 kDa. The 10-kDa group was resolved by anion-exchange chromatography into four fractions while the 20-kDa group was resolved into three fractions using this method. After metal removal and S-methylation of the cysteine residues using methyl-p-nitrobenzenesulphonate the complete amino acid sequences were determined. Five isoforms of the 20-kDa group were shown to possess monomeric units consisting of 71 amino acids. These proteins were distinct from the four 72-amino-acid proteins of the 10-kDa group. The FASTA algorithm has been used to compare the degree of similarity between the mussel metallothionein MT-10-IV isoform and other metallothioneins. The mussel MT-10-IV isoform exhibited substantial similarity to other molluscan metallothioneins. Moreover, the mussel metallothionein exhibited more similarity to vertebrate metallothioneins than to those of non-molluscan invertebrates, thus suggesting that the mussel metallothioneins are class I metallothioneins.
Extracted Claims
3 claims extracted from this paper into the knowledge graph
mussel metallothionein MT-10-IV isoform exhibits substantial similarity
“The mussel MT-10-IV isoform exhibited substantial similarity to other molluscan metallothioneins.”
cadmium induces metallothioneins
“Cadmium-induced metallothioneins from the common sea mussel, Mytilus edulis, were shown to comprise of two groups of isoforms having apparent molecular masses of 10 kDa and 20 kDa.”
mussel metallothionein exhibits more similarity
“Moreover, the mussel metallothionein exhibited more similarity to vertebrate metallothioneins than to those of non-molluscan invertebrates, thus suggesting that the mussel metallothioneins are class I...”