Application of Electrospray Ionization Mass Spectrometry to the Study of Grape Anthocyanins
Donata Favretto, Riccardo Flamini
American Journal of Enology and Viticulture
Abstract
A rapid method for the qualitative and semi-quantitative determination of grape anthocyanins has been developed. It consists of extraction of anthocyanins from grape skins with an ethanol/water/hydrochloric acid solution, purification and concentration by solid phase extraction, and analysis of extracts by direct infusion electrospray mass spectrometry and ion trap multiple mass spectrometry. Two different cultivars of hybrid grapes and a cultivar of <i>Vitis vinifera</i> grape were analyzed and they showed different anthocyanin profiles. Among the compounds detected and characterized in the extracts, four anthocyanin-3-(6-O-<i>p</i>-coumaroyl)5-O-diglucosides were identified in Clinton grape for the first time and their electrospray ionization mass spectra could be obtained for the first time together with those of cyanidin, peonidin, and malvidin 3,5-O-diglucosides. For malvidin 3,5-O-diglucoside and 3-O-monoglucoside a semi-quantitative determination could be achieved that would result interesting for the identification of hybrid grapes and wines.
Extracted Claims
6 claims extracted from this paper into the knowledge graph
peonidin is identified in grape extracts
“Among the compounds detected and characterized in the extracts, four anthocyanin-3-(6-O-p-coumaroyl)5-O-diglucosides were identified in Clinton grape for the first time and their electrospray ionizati...”
malvidin 3,5-O-diglucoside is semi-quantitatively determined grape extracts
“For malvidin 3,5-O-diglucoside and 3-O-monoglucoside a semi-quantitative determination could be achieved that would result interesting for the identification of hybrid grapes and wines.”
anthocyanin-3-(6-O-p-coumaroyl)5-O-diglucosides are identified in Clinton grape
“Among the compounds detected and characterized in the extracts, four anthocyanin-3-(6-O-p-coumaroyl)5-O-diglucosides were identified in Clinton grape for the first time”