Formate Dehydrogenase of <i>Clostridium thermoaceticum</i> : Incorporation of Selenium-75, and the Effects of Selenite, Molybdate, and Tungstate on the Enzyme
Jan R. Andreesen, Lars G. Ljungdahl
Journal of Bacteriology
Abstract
The formation of the nicotinamide adenine dinucleotide phosphate-dependent formate dehydrogenase in Clostridium thermoaceticum is stimulated by the presence of molybdate and selenite in the growth medium. The highest formate dehydrogenase activity was obtained with 2.5 x 10(-4) M Na(2)MoO(4) and 5 x 10(-5) Na(2)SeO(3). Tungstate but not vanadate could replace molybdate and stimulate the formation of formate dehydrogenase. Tungstate stimulated activity more than molybdate, and in combination with molybdate the stimulation of formation of formate dehydrogenase was additive. Formate dehydrogenase was isolated from cells grown in the presence of Na(2) (75)SeO(2), and a correlation was observed between bound (75)Se and enzyme activity.
Extracted Claims
4 claims extracted from this paper into the knowledge graph
selenite stimulates formate dehydrogenase formation
“The highest formate dehydrogenase activity was obtained with 2.5 x 10(-4) M Na(2)MoO(4) and 5 x 10(-5) Na(2)SeO(3).”
tungstate replaces molybdate
“Tungstate but not vanadate could replace molybdate and stimulate the formation of formate dehydrogenase.”
tungstate stimulates formate dehydrogenase formation
“Tungstate stimulated activity more than molybdate, and in combination with molybdate the stimulation of formation of formate dehydrogenase was additive.”