Oxygen requirements of yeasts
Wouter F. Visser, W. A. Scheffers, Wilma H. Batenburg-van der Vegte, Johannes P. van Dijken
Applied and Environmental Microbiology
Abstract
Type species of 75 yeast genera were examined for their ability to grow anaerobically in complex and mineral media. To define anaerobic conditions, we added a redox indicator, resazurin, to the media to determine low redox potentials. All strains tested were capable of fermenting glucose to ethanol in oxygen-limited shake-flask cultures, even those of species generally regarded as nonfermentative. However, only 23% of the yeast species tested grew under anaerobic conditions. A comparative study with a number of selected strains revealed that Saccharomyces cerevisiae stands out as a yeast capable of rapid growth at low redox potentials. Other yeasts, such as Torulaspora delbrueckii and Candida tropicalis, grew poorly mu max, 0.03 and 0.05 h-1, respectively) under anaerobic conditions in mineral medium supplemented with Tween 80 and ergosterol. The latter organisms grew rapidly under oxygen limitation and then displayed a high rate of alcoholic fermentation. It can be concluded that these yeasts have hitherto-unidentified oxygen requirements for growth.
Extracted Claims
5 claims extracted from this paper into the knowledge graph
yeasts have oxygen requirements
“It can be concluded that these yeasts have hitherto-unidentified oxygen requirements for growth.”
Torulaspora delbrueckii and Candida tropicalis grow rapidly
“The latter organisms grew rapidly under oxygen limitation and then displayed a high rate of alcoholic fermentation.”
Torulaspora delbrueckii and Candida tropicalis grow poorly
“Other yeasts, such as Torulaspora delbrueckii and Candida tropicalis, grew poorly (mu max, 0.03 and 0.05 h-1, respectively) under anaerobic conditions in mineral medium supplemented with Tween 80 and ...”