An Agar Dilution Method for the Determination of the Minimum Inhibitory Concentration of Essential Oils
Shane G. Griffin, Julie L. Markham, David N. Leach
Journal of Essential Oil Research
Abstract
Abstract Methods for the measurement of the minimum inhibitory concentration (MIC) of antibacterial agents have been developed for water-soluble substances and require adaptation for use with water-insoluble essential oils. This paper reports a standardized agar dilution MIC method, using 0.5% v/v Tween 20 as a dispersant, which provides a reliable and reproducible technique. The method was tested using two Melaleuca alternifolia oil samples with two different levels of terpinen-4-ol (37% and 45% v/v). The MIC values of die tea tree oil samples against a wide selection of bacteria, moulds and yeast are reported.
Extracted Claims
3 claims extracted from this paper into the knowledge graph
agar dilution method uses 0.5% v/v Tween 20 as a dispersant
“This paper reports a standardized agar dilution MIC method, using 0.5% v/v Tween 20 as a dispersant, which provides a reliable and reproducible technique.”
Melaleuca alternifolia oil has different levels of terpinen-4-ol (37% and 45% v/v)
“The method was tested using two Melaleuca alternifolia oil samples with two different levels of terpinen-4-ol (37% and 45% v/v).”
Melaleuca alternifolia oil inhibits bacteria, moulds, and yeast
“The MIC values of the tea tree oil samples against a wide selection of bacteria, moulds and yeast are reported.”