Yeast Aldehyde Dehydrogenase
Charles R. Steinman, William B. Jakoby
Journal of Biological Chemistry
Abstract
The potassium-activated, pyridine nucleotide-linked aldehyde dehydrogenase from yeast has been purified to the stage of homogeneity as judged by ultracentrifugation and gel electrophoresis.The enzyme has been crystallized, although this is not a recommended step in purification because loss of catalytic activity is thereby incurred.At least three separable, active fractions were obtained with the large-scale purification procedure presented in this report.However, only one major fraction was found when precautions were taken to minimize proteolysis.The purified aldehyde dehydrogenase has a molecular weight of 200,000.
Extracted Claims
6 claims extracted from this paper into the knowledge graph
aldehyde dehydrogenase has molecular weight of 200,000
“The purified aldehyde dehydrogenase has a molecular weight of 200,000.”
potassium activates pyridine nucleotide-linked aldehyde dehydrogenase
“The potassium-activated, pyridine nucleotide-linked aldehyde dehydrogenase from yeast has been purified to the stage of homogeneity as judged by ultracentrifugation and gel electrophoresis.”
gel electrophoresis judges homogeneity
“The potassium-activated, pyridine nucleotide-linked aldehyde dehydrogenase from yeast has been purified to the stage of homogeneity as judged by ultracentrifugation and gel electrophoresis.”