What You Need to Know
The 100 °C heat denatures enzymes that cause spoilage and kills vegetative bacteria, while the acidity (pH ≤ 4.6) inhibits the growth of Clostridium botulinum spores. The process relies on uniform heat transfer in a water bath and precise time‑temperature control to ensure safety.
The Science
Primary Reaction
Thermal inactivation of vegetative microbes and enzymes; acid‑mediated inhibition of Clostridium botulinum spores