Isolation and characteristics of a skatole-producing Lactobacillus sp. from the bovine rumen
M. T. Yokoyama, James R. Carlson, Lillian V. Holdeman
Applied and Environmental Microbiology
Abstract
A bacterium that is capable of decarboxylating indoleacetic acid to skatole (3-methylindole) has been isolated from an L-tryptophan enrichment of bovine rumen fluid. The bacterium is a gram-positive, nonmotile, nonsporeforming rod. It is an obligate anaerobe, and strains predominatly produce D-(-)-lactic acid, with smaller amounts of L-(+)-lactic acid and acetic acid, from sugar. All four strains isolated gave a negative reaction to the indole test because they cannot form skatole directly from tryptophan. This is the first report of indoleacetic acid decarboxylation to skatole in pure culture and the demonstration of skatole production by a Lactobacillus species.
Extracted Claims
3 claims extracted from this paper into the knowledge graph
Lactobacillus sp. cannot form skatole directly from tryptophan
“All four strains isolated gave a negative reaction to the indole test because they cannot form skatole directly from tryptophan.”
Lactobacillus sp. is capable of decarboxylating indoleacetic acid to skatole
“A bacterium that is capable of decarboxylating indoleacetic acid to skatole (3-methylindole) has been isolated from an L-tryptophan enrichment of bovine rumen fluid.”
Lactobacillus sp. produces D-(-)-lactic acid, L-(+)-lactic acid, and acetic acid
“It is an obligate anaerobe, and strains predominatly produce D-(-)-lactic acid, with smaller amounts of L-(+)-lactic acid and acetic acid, from sugar.”